anticd80  (Bioss)

Journal: iScience

Article Title: Synergistic immunomodulatory effects of naringenin and photothermal therapy in triple-negative breast cancer

doi: 10.1016/j.isci.2025.114124

Figure Lengend Snippet: Network pharmacological analysis (A) Venn diagram showing the numbers of the overlapping genes between NRG and TNBC. (B) The PPI network of the overlapping targets. (C) GO enrichment analysis of the biological process overlapping targets. (D) Representative flow cytometry plots of CD80 + CD86 + expression in CD11c + cells from tumor-draining lymph nodes of 4T1-tumor-bearing mice treated with different regimens. (E) Quantitative analysis of CD80 + CD86 + expression in CD11c + cells. Data are presented as mean ± SD. ∗ p < 0.05, ∗∗ p < 0.01, ∗∗∗ p < 0.001, ns p ≥ 0.05. Data are represented as mean ± SD, and all experiments were conducted with n = 5 animals per group.

Article Snippet: CD80 Polyclonal antibody , Proteintech , 14292-1-AP; RRID: AB_10640809.

Techniques: Flow Cytometry, Expressing

Journal: Biomedicines

Article Title: Impaired Development of Collagen Antibody-Induced Arthritis in Rab44-Deficient Mice

doi: 10.3390/biomedicines12112504

Figure Lengend Snippet: mRNA levels of arthritis marker genes in the ankle joints of mice. mRNA was extracted from the ankle joints of wild-type (WT) control (w−), WT collagen antibody-induced arthritis (CAIA) (w+), Rab44-knockout (KO) control (k−), and Rab44-KO CAIA mice (k+). Subsequently, qRT-PCR was performed. ( a ) Inflammatory markers, tumor necrosis factor-α ( Tnfa ), interleukin-1β ( Il1b ), interleukin-6 (Il6), and CD80 ( Cd80 ). ( b ) Cartilage turnover markers collagen type Iα 1 chain ( Col1a1 ), collagen type IIα 1 chain ( Col2a1 ), SRY-Box transcription factor 9 ( Sox9 ), aggrecan ( Acan ) and matrix metalloprotease 13 ( Mmp13 ). ( c ) Bone formation markers, Sp7 transcription factor ( Sp7 ) and RUNX family transcription factor 2 ( Runx2 ). ( d ) Bone resorption markers, tartrate-resistant acid phosphatase type 5 (Acp5) and cathepsin K ( Ctsk ). (* p < 0.05, ** p < 0.01).

Article Snippet: The CD80 polyclonal antibody (Cat. no. bs-2211R) was purchased from Bioss (Woburn, MA, USA).

Techniques: Marker, Control, Knock-Out, Quantitative RT-PCR

Journal: Biomedicines

Article Title: Impaired Development of Collagen Antibody-Induced Arthritis in Rab44-Deficient Mice

doi: 10.3390/biomedicines12112504

Figure Lengend Snippet: Immunohistochemical analysis of M1- and/or M2-type macrophages in the joint of collagen antibody-induced arthritis (CAIA) mice. ( a ) The fixed sections of wild-type (WT) and Rab44-knockout (KO) CAIA mice were blocked with 1.0% skim milk in PBS. The samples were incubated with rabbit anti-CD68 IgG (total macrophage marker), CD80 IgG (M1-type macrophage marker) and CD206 IgG (M2-type macrophage marker) as the primary antibodies, followed by an HRP/DAB detection method. Samples were observed under an optical microscope. Bars: 100 μm. ( b ) Quantitative analysis of the serial sections with the number of CD68-positive cells visualized in a certain field (left panel), the percentage of the number of CD80-positive cells in the number of CD68-positive cells (middle panel), and the percentage of the number of CD206-positive cells in the number of CD68-positive cells (right panel). Data are represented as the mean ± S.E. of results from four independent experiments. Asterisks indicate statistical significance compared to the control; ( n = 4, * p < 0.05, ** p < 0.01).

Article Snippet: The CD80 polyclonal antibody (Cat. no. bs-2211R) was purchased from Bioss (Woburn, MA, USA).

Techniques: Immunohistochemical staining, Knock-Out, Incubation, Marker, Microscopy, Control